| |
Fig. 2. Cellular localization of BDNF and trkB mRNA in the vestibuloauditory ganglion cells. Sections a,b were treated with BDNF or trkB
sense riboprobes. Sections c-f were hybridized with BDNF or trkB antisense riboprobes, a,b: Darkfield photomicrographs depict sense riboprobe
controls for (a) BDNF and (b) trkB. Note the lack of specific hybridization. Scale bar= 50 ~m. c: Darkfield photomicrograph displays
BDNF mRNA in the vestibuloauditory ganglion. Scale bar= 50 l-tm. d: Darkfield photomicrograph illustrates similar expression levels of trkB
mRNA in the vestibuloauditory ganglion. Scale bar = 50 ~tm. e: Brightfield photomicrograph at higher magnification demonstrates BDNF transcripts
concentrated over neuron somata (arrowheads). Scale bar= 12 ~tm. f: Brighfield photomicrograph at higher magnification shows neuronal
cell bodies (arrowheads) containing trkB transcripts. Scale bar = 12 gm. |
