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bdnfxenopus retinal inner nuclear layer 

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Experiment details for bdnf

The cellular patterns of BDNF and trkB expression suggest multiple roles for BDNF during Xenopus visual system development.

The cellular patterns of BDNF and trkB expression suggest multiple roles for BDNF during Xenopus visual system development.

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Gene Clone Species Stages Anatomy
bdnf.L laevis NF stage 40 retinal inner nuclear layer

  FIG. 3. Expression of BDNF mRNA in the developing Xenopus retina. Transverse sections through stage 40 (A) and 45 (B and C) retinas hybridized in situ with a digoxygenin-labeled antisense Xenopus BDNF probe show specific cytoplasmic hybridization signals over cell bodies in the ganglion cell layer (gcl) and the ciliary margin cells (cm). In some stage 45 retinal sections, one to two digoxygenin-labeled cells were observed in the inner nuclear layer (inl) (B; arrowhead). Note the increase in both the intensity and number of digoxygeninpositive cells in the gcl of stage 45, versus the stage 40, retina. Due to the conditions of the hybridization procedure (see Materials and Methods), the specific cytoplasmic hybridization signal is excluded from the large cell nuclei. (D) Hybridization of a stage 45 retina with a sense BDNF probe shows no hybridization signal. ipl, inner plexiform layer; onl, outer nuclear layer; opl, outer plexiform layer; pe, pigmented epithelium. Dorsal is up, ventral is down. Scale bar, 50 mm.
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