|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
bmp4
|
|
laevis
|
NF stage 32
|
otic vesicle
,
retina
,
heart
,
ciliary marginal zone
,
pharyngeal arch
,
[+]
|
bmp4
|
|
laevis
|
NF stage 26
to
NF stage 28
|
otic vesicle
,
retina
,
eye
,
dorsal
,
heart primordium
,
[+]
|
|
|
Fig. 1. Bmp4 mRNA, but not Bmp2, is expressed in the forming Xenopus retina. (A,B) Spatial expression of Bmp2 mRNA in Xenopus embryos processed for wholemount in situ hybridization using digoxige- nin-labeled riboprobe. Lateral views of stage 26/28 (A) and stage 32 (B) embryos. (C,D) Expression of Bmp4 mRNA in wholemount embryos at stages 26/28 (C) and 32 (D). (E,F,H) Transverse sections through retinas of embryos processed for Bmp4 in situ hybridization at stages 26/28 (E), 32 (F) and 35/36 (H). (G) Immunolabeling of BrdU that has incorporated into dividing cells at stage 33/34 shows the location of the ciliary marginal zone (CMZ; white arrowheads), a proliferative region adjacent to the lens. Compare with Bmp4 expression (black arrowhead) at stage 32 (F). Anterior is to the left in panels (A-D). Scale bar in (E) is 50 μm for (E-H). Abbreviations: D, dorsal; V, ventral; ba, branchial arches; e, eye; h, heart; L, lens; nr, neural retina; p, pineal gland; ol, olfactory pits; ot, otic vesicle; rpe, retinal pigmented epithelium. |