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FIG. 8. BMP4 is expressed in the non-neural ectoderm and both BMP4 and 7 are expressed in the prechordal mesoderm, underlying the anteriormost neural plate. (A) Anterior view of a stage 17 embryo stained by whole-mount in situ hybridization to BMP4 (purple stain). BMP4 is expressed around and bordering the keyhole-shaped neural plate with stronger expression anteriorly. The dotted white line represents the angle of sectioning shown in B, C, and D. (B and C) Sagittal section through an embryo stained for BMP4 mRNA, anterior is to the left. Low power (B) and high power (C) reveal the expression of BMP4 in the prechordal meso- derm (arrow). (D) Sagittal section through a stage 17 embryo stained by whole-mount in situ hybridization to BMP7 mRNA. The anterior-most portion of the section is shown. BMP7 is expressed in the prechordal mesoderm (arrow), underlying the prechordal plate. |
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FIG. 9. (A) Injection of XBF-1 RNA suppresses BMP4 expression in the ectoderm. XBF-1 was coinjected with lacZ RNA (blue). (B) Animal caps combined with BSA beads express a basal level of msx1 (left panel). Animal caps combined with BMP4-soaked beads express a higher level of msx1 (middle panel). Animal caps injected with XBF-1 mRNA and combined with BMP4 beads show reduced msx1 expression (right panel). |