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Figure S2. Xenopus Admp Overexpression Activates Known BMP Target Genes (A) Vent-1, a direct target of BMP signaling, is expressed in the ventral part of the closing blastopore at stage 12. (B) Xenopus Admp mRNA injection (25 pg in each blastomere at the 4-cell stage) up-regulates Vent-1 expression over the entire surface of the embryo. (C) Bmp4 expression is localized at stage 12 in the ventral part of the closing blastopore (or ventral center). (D) Admp mRNA overexpression turns on Bmp4 transcription uniformly indicating that Bmp4 is under positive feedback regulation by BMP-like signals. (E) MyoD, which marks future somites, is expressed at stage 12 as a mesodermal ring around the closing blastopore with exception of the future notochord. (F). Admp mRNA overexpression eliminates notochord differentiation leaving a radial ring of MyoD expression around the closing blastopore. These experiments indicate that ADMP has similar phenotypic effects to those of other BMP signals. |