| |
SF1.1 Gene expression analysis of MEK1-depleted gastrula embryos.
(A–D) Four-cell embryos were injected in each blastomere with 25 ng Mk-MO, collected at early gastrula stage 10.5 and processed for RT-qPCR to quantify changes in the expression levels of pro-differentiation markers (A), or changes in the expression levels of BMPs (Smad1/5/8) and Activin/Nodal (Smad2/3) signaling targets (C). For all qPCR graphs, error bars represent s.e.m. values of four independent experiments with two technical duplicates. For statistical analyses, samples from injected embryos were compared with samples from uninjected control embryos by Unpaired Student’s t-test. *p<0.05, **p<0.005, ***p<0.0001. (B,D) Some embryos from the same experiment were processed for WISH with the indicated probes. Note that embryos stained for xk81a1 (epidermis) were injected with 25 ng Mk-MO in one ventral animal blastomere at 16-cell stage and collected at late gastrula stage 13. In B and D, the number of embryos exemplified by the photograph over the total number of embryos analyzed is indicated. |
