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Figure 2. Differentiation of anteroventral cells is regulated by BMP and Notch. Anterior (A-D,I,J,L,N,O), lateral (E-H), or dorsal (K,M,P) views of neurula to early tail bud embryos are shown. A,B: Stage 13 embryo stained with BMP4 alone (A) or double stained with Phox2a (arrowhead) and BMP4 (B). C,D: Embryos injected with tBR mRNA show a reduction of Phox2a and tyrosine hydroxylase (TH) expression in the anteroventral region. E-H: Induction of Phox2a and TH by Xash1 is reduced by coinjection of tBr. I: Delta1 is transiently expressed in the anteroventral cells (arrowhead). J,K: Embryos injected with Notch1 ICD or Su(H)Ank show a decrease of IA1 expression in the neural plate primary neurons and anteroventral cells (arrowheads). L,M: Embryos injected with Delta1stu or Su(H)DBM mRNA show an increase of IA1 expression in the neural plate primary neurons, trigeminal placodes, and anteroventral cells (arrowheads). |
