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Fig. 6. Gene expression detection in dkk1 heat shock and small molecule treated tadpole tails. (A–F) In situ hybridization detection of fgf10 (A, D), fgf20 (B, E), msx1(C, F) in tadpoles treated with DMSO alone (A–C) or 50 nM BIO (D–F). (G–L) In situ hybridization detection of fgf8 (G), fgf 9 (H), fgf10 (I), fgf20 (J), msx1 (K), msx2 (inset in panel K) and Bmp4 (L) in dkk1 transgenic tadpole tails with heat shock treatment, 3 days post amputation. (M–O) In situ hybridization detection of wnt3a (M), wnt5a (N) and Bmp4 (O) in wild type tadpole tails treated with 100 μM SU5402, 3 days post amputation. Arrowheads indicate amputation levels. Scale bar: 500 μm. |
