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bmp4xenopus   

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Experiment details for bmp4

Gessert S et al. (2008) Assay

DM-GRASP/ALCAM/CD166 is required for cardiac morphogenesis and maintenance of cardiac identity in first heart field derived cells.

Gene Clone Species Stages Anatomy
bmp4.L laevis NF stage 28 heart , cardiac mesoderm , heart primordium

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  Fig. 5. Loss of DM-GRASP affects marker gene expression in the FHF but not the SHF. (A) DM-GRASP MO was unilaterally injected at the 8-cell stage and expression of cardiac marker genes was monitored at stage 20. No changes for Tbx20, Isl-1 and Nkx2.5 could be detected. Anterior views of whole embryos are given. (B) DM-GRASP MO was unilaterally injected at the 8-cell stage and expression of cardiac marker genes as indicated was monitored at stage 28. Loss of DM-GRASP resulted in down-regulation of some marker genes of the FHF (arrows) but not SHF such as Isl-1 or BMP-4. Ventral views of embryos are given. (C) Quantitative presentation of observed phenotype in panel B. Error bars are standard errors of the means. N = number of examined embryos, n = number of independent experiments.