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cyp26b1xenopus hindlimb digit 2 

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Experiment details for cyp26b1

Expression of key retinoic acid modulating genes suggests active regulation during development and regeneration of the amphib...

Expression of key retinoic acid modulating genes suggests active regulation during development and regeneration of the amphibian limb.

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Gene Clone Species Stages Anatomy
cyp26b1.L laevis NF stage 54 hindlimb digit 2

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  Figure 4. Cyp26b expression is distal in early limbs and restricted to the forming autopod skeleton and claws later. Cyp26b expression in hindlimbs (h) and forelimbs (f). Limbs are oriented with proximal to the left and posterior uppermost viewed from the dorsal side except L, which is a ventral view. A:Cyp26b is not expressed in early st. 51 hindlimbs. B, H: Strong expression (white asterisk) in the distal half of mesenchyme is seen by late st.51/early st. 52 hindlimbs (B) and st. 52 forelimbs (H) with the exception of the distal-most cells. From st. 52, the extent of expression in the most distal mesenchyme cells underlying the AER is marked by black arrows (C, H). Note that this never reaches as far as digit V. A more proximal domain of expression appears at stage 52 in hindlimbs (yellow asterisk in C). D: Yellow arrows mark expression in metatarsals in st. 53 hindlimbs. I: St. 53 forelimbs show no expression of Cyp26b in the autopod. E: Expression in hindlimb metatarsals of digits IV and II and phalanges of digits V and IV at st. 54 (yellow arrows). J: Forelimbs at st. 54 show no expression of Cyp26b in the autopod. F:Cyp26b is expressed in the most distal mesenchyme of toes I, II, and III (black arrowheads) but is absent from toes IV and V and from forelimb digits (K). G: At stage 56, Cyp26b is expressed in forming claws of toes I–III (arrowheads) and in distal most phalanges of digits I–IV (yellow arrows). L: Ventral view of st. 56 forelimb reveals expression in all four digits.
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