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Fig. 1. (A) Whole-mount in situ hybridization of oocytes at
stages I–VI with antisense or sense ribonucleic acid (RNA) probe
for XVLG1 RNA. A signal representing XVLG1 RNA is clearly seen
in stage I–III oocytes but nearly undetectable in stage IV–VI
oocytes with the antisense probe (upper tier). The signal is not
detected in oocytes at any stage when the sense probe is
employed (lower tier). From left to right, oocytes at stages from
I to VI are lined up in order. (B) A section of the whole-mount
specimen of stage I–III oocytes hybridized with the antisense
probe as shown in Fig. 1(A). XVLG1 RNA is distributed throughout
the cytoplasm of stage I–III oocytes, except for the mitochondrial
cloud (arrow) of the stage I oocyte. It seems to
disappear with oocyte growth, but can be still observed moderately
in stage II oocytes and faintly in stage III oocytes. Bar,
1 mm (A), 100 μm (B). |
