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Figure 1. Expression of Dicer in Xenopus tadpole limbs. (A, B) Dicer transcripts detected by RT-PCR and real-time PCR. Relative
expression of Dicer was normalized to Ef1a and compared to stage 53 unamputated limb (St53C). RT–: no reverse transcriptase
control; St33: stage 33 tailbud stage embryo; St53R: stage 53 limb regenerate, 4 days postamputation (dpa); St57C:
stage 57 unamputated hindlimb, distal part; St57R, stage 57 limb stump, 8 dpa. Result in (B) was from two independent
experiments. (C, D) Dicer transcript detection by in situ hybridization in the limb stumps of stage 53, 4 dpa (C, lateral view),
and stage 57, 8 dpa (D, ventral view) tadpoles. In situ signals are in purple blue. Black dots in (D) are melanocytes. (E–G)
Immunofluorescence analysis of Dicer expression in parasagittal sections of unoperated stage 53 limb (E) and regenerating
limb, 4 dpa (F). (G) Enlarged view of Dicer expression in the regenerating blastemal. Scale bars: 0.5 mm in (C, D); 0.1 mm in
(E–G). dpa: days postamputation. White arrowheads in (C, D, F) indicate amputation planes. |
