|
Gene |
Clone |
Species |
Stages |
Anatomy |
dlc.L
|
|
laevis
|
NF stage 32
|
brain
,
forebrain
,
telencephalon
,
diencephalon
,
lateral ganglionic eminence
,
[+]
|
dlc.L
|
|
laevis
|
NF stage 35 and 36
|
brain
,
forebrain
,
telencephalon
,
diencephalon
,
optic stalk
,
[+]
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
eomes
|
|
laevis
|
NF stage 32
|
lateral ganglionic eminence
,
pallium
,
dorsal pallium
,
lateral pallium
,
medial pallium
,
[+]
|
eomes
|
|
laevis
|
NF stage 35 and 36
|
lateral ganglionic eminence
,
pallium
,
dorsal pallium
,
lateral pallium
,
medial pallium
,
[+]
|
pax6
|
|
laevis
|
NF stage 32
|
spinal cord
,
lateral ganglionic eminence
,
pallium
,
zona limitans intrathalamica
,
lateral pallium
,
[+]
|
pax6
|
|
laevis
|
NF stage 35 and 36
|
lateral ganglionic eminence
,
pallium
,
pretectum
,
prethalamus
,
zona limitans intrathalamica
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 32
|
brain
,
forebrain
,
telencephalon
,
diencephalon
,
optic stalk
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 35 and 36
|
telencephalon
,
optic stalk
,
hypothalamus
,
anterior hypothalamus
,
posterior hypothalamus
,
[+]
|
emx1
|
|
laevis
|
NF stage 32
|
diencephalon
,
dorsal pallidum
,
lateral pallium
,
medial pallium
,
pallidum
,
[+]
|
emx1
|
|
laevis
|
NF stage 35 and 36
|
diencephalon
,
pallium
,
zona limitans intrathalamica
,
thalamus
,
dorsal pallium
,
[+]
|
|
|
Fig.1. In toto analysis of x-Dll3, x-Nkx2.1, x-Emx1, x-Pax6 and x-Eomes expression in Xenopus brain. Whole-mount dissected brains at stage 32 (left column, entire brain) and stage 35 (right column, focused on the forebrain) are photographed in lateral views. In all panels, anterior is left and dorsal is up. The arrowhead in (A,C,E,G,I) indicates the position of the psb between the pallium (pa) and the subpallium (spa). The asterisk in (B) points to the x-Dll3 negative area in the rostral forebrain, see text. The inset indicates the plan of sections for transverse (t) and horizontal (h) sections in the next figures, as deduced from the axis of the brain. This antero-posterior axis is indicated by black arrows. Therefore, in the telencephalon, a horizontal section is parallel to the axis and allows the visualisation of transverse boundaries and divisions. Abbreviations are in Table 1. Scale BAR=100 μm. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
eomes
|
|
laevis
|
NF stage 40
|
forebrain
,
telencephalon
,
pallium
,
dorsal pallium
,
lateral pallium
,
[+]
|
pax6
|
|
laevis
|
NF stage 40
|
lateral ganglionic eminence
,
pallium
,
dorsal pallium
,
ventral pallium
,
prosomere
,
[+]
|
emx1
|
|
laevis
|
NF stage 40
|
medial ganglionic eminence
,
pallium
,
dorsal pallium
,
ventral pallium
,
prosomere
|
|
|
Fig. 3. Relative expression of x-Dll3, x-Emx1, x-Pax6 and x-Eomes at stage 40. Expression of pallial markers (blue) relative to x-Dll3 (orange) on horizontal (A,B) and transverse (C) sections through the forebrain of stage 40 embryos. Horizontal sections and transverse sections are presented in a rostro-caudal and dorso-ventral order, respectively. Asterisk in (A) indicates the x-Dll3 negative area surrounded by the subpallium (same as Fig.1 and Fig. 2). In all panels, arrowheads indicate the presence of pallial markers expressed in a subpallial (x-Dll3 expressing) location. See Table 1 for abbreviations. Scale BAR=100 μm. |