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Fig. 9. Hox PG1 genes are necessary for somitogenesis and X-Delta-2 expression. Morpholinos for each of the Hox PG1 genes (Hoxa1, -b1, and -d1) were injected in combination into the LHS of the embryo (PG1 MO, C,F). Non-injected controls (Nic; A,D) and embryos injected with control MO (B: 100%, n=31; E: 100%, n=23) are also shown. The expression of the muscle precursor marker MyoD (A-C) and of X-Delta-2 (D–F) was analysed at late tailbud stage (stage 25). Embryos are shown from a lateral view, with the anterior side to the left. Loss of PG1 function perturbs somite formation (C: 76%, n=47) and downregulates X-Delta-2 expression in the somitomeric region (F: 79%, n=29). Arrow in (F) indicates X-Delta-2 expression in the somitomeric region. |
