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Figure 1. Analysis of δNp63 expression in developing embryos. A: Analysis of δNp63 temporal expression pattern. Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from embryos at different embryonic stages. EF1α amplification was used as control. B–P: Whole-mount in situ hybridization analysis of spatiotemporal δNp63 expression. B: Lateral view. C,D,J–L: Lateral view, anterior on the right, dorsal at the top. E,M–P: Anterior view, dorsal at the top. F,H,I: Dorsal view, anterior on the right. G,M′–P′: Transversal sections. B,F,G,H: In situ hybridization for δNp63. C,D: Double in situ hybridization for δNp63 (purple) and XK81a (turquoise). G: Small arrow, δNp63 expression in the internal layer of the ectoderm. Inset, δNp63 (purple) and XK81a (turquoise). (H) Small arrow, two medial stripes of δNp63 expression. E,N,N′: δNp63 (purple) and Sox2 (turquoise). I,J,M,M′: δNp63 (purple) and FoxD3 (turquoise). L,P,P′: δNp63 (purple) and Dlx5 (turquoise). K, O, O′: In situ hybridization for Six1. Asterisk, δNp63 expression in the prospective epidermis. Red star, ventral anterior prospective epidermis with no δNp63 expression. Arrowhead, δNp63 expression in the most external limit of the neural crest overlapping with Six1 and Dlx5 expression. Large arrow, anterior δNp63 expression overlaps with Six1 and Dlx5. i.l., internal layer; e.l., external layer. An, animal; Vg, vegetal; D, dorsal; V, ventral; np, neural plate.Download figure to PowerPoint |
