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Fig. 7. Effect of XPtc1 Loop2 and cyclopamine treatment on otic specification. (A-E) Embryos were injected with XPtc1 Loop2 mRNA (750 pg) into one
of two cells, fixed at stage 36, and stained for Xdll-3 expression. Embryos injected with XPtc1 Loop2, a dominant-negative form of XPtc1, exhibit ectopic
vesicular structures expressing Xdll-3 (31%, n 36) (indicated by arrows) and enlarged otic vesicles (E). (F) Normal-sized otic vesicle in control embryo
treated with 0.38% ethanol (100%, n 10). (G) Enlarged otic vesicle developed upon treatment with 20 M cyclopamine (50%, n 8). Con, control; cyc,
cyclopamine; is, injected side; nis, noninjected side. |
