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Fig. 1. Spatial expression patterns of Dlx5 and Dlx6. Embryos were fixed and hybridized under conditions optimized for detection of epidermal transcripts. (A-E), Dlx5. (F-J), Dlx6. (A,F) Mid/ late gastrula (stage 12) showing broad expression in ectoderm, except for the dorsal side (on the right) which is the posterior region of the prospective neural plate. The two black arrowheads indicate the edges of the yolk plugs. (B,G) Early neurula (stage 13). A stripe of relatively intense hybridization has appeared (white arrows in these and subsequent panels) lateral to the epidermal-neural plate boundary (black arrow). The yolk plugs are indicated by black arrowheads. Dorsal is to the right. (C,H) Mid neurula (stage 15). Anterior neural plate hybridization has resolved into anterior neural fold and cement gland (black arrowheads). Anterior is to the right. (D,I) Late neurula (stage 17). The intense hybridization stripes (white arrows) and epidermal-neural plate boundaries (black arrows) continue to be well-resolved. Anterior to right. (E,J) Early tailbud (stage 20). The neural tube has closed at this stage and the epidermal-neural plate boundaries have fused at the dorsal midline. The intense lateral stripe of expression clearly lies outside the neural tube for both Dlx5 and Dlx6 (white arrows). Anterior is to the right. |
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Fig. 2. Width of dorsal excluded regions for Dlx genes. Stage-matched (st 14) embryos hybridized in situ with probes for chordin (blue/green) and Dlx3, 5 or 6 (purple). (A,D) Dlx3; (B,E) Dlx5; (C,F) Dlx6. The patterns are similar, but the distance between the midline (chordin signal) and Dlx3 is clearly greater than for either Dlx5 or Dlx6. A-C, dorsal view. D-F, lateral view. |