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FIG. 3. Pcl1 effects on brain and eye markers. Control (b-galactosidase) or mPcl1 RNA was injected bilaterally into the dorsal marginal
zone of four-cell-stage embryos. Expression of N-CAM (pan-neural), Rx2A (eye), Xotx2 (eye, forebrain, midbrain), X-dll3 (forebrain, otic
vesicle, branchial arches, cement gland), and Xwnt8-b (forebrain–midbrain boundary) was assayed by whole-mount RNA in situ
hybridization. Representative embryos are shown from Stage 19–22 (left) and Stage 26 (right). Developing eye field is denoted by
arrowheads, and forebrain-specific gene expression is denoted by arrows. Note that neural patterning is unchanged at Stage 19–22, even
when eye markers are completely absent. |
