|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
twist1
|
|
tropicalis
|
NF stage 24
|
hyoid crest
,
neural crest
,
trunk neural crest
,
cranial neural crest
,
migratory neural crest cell
|
twist1
|
|
tropicalis
|
NF stage 28
|
neural crest
,
branchial arch
,
cranial neural crest
,
migratory neural crest cell
|
twist1
|
|
tropicalis
|
NF stage 14
|
neural crest
,
cranial neural crest
|
sox3
|
|
tropicalis
|
NF stage 14
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
gsc
|
|
tropicalis
|
NF stage 28
|
neural crest
,
branchial arch
,
cranial neural crest
|
snai2
|
|
tropicalis
|
NF stage 24
|
mandibular crest
,
hyoid crest
,
neural crest
,
cranial neural crest
,
migratory neural crest cell
|
sox9
|
|
tropicalis
|
NF stage 28
|
retina
,
mandibular crest
,
neural crest
,
eye
,
optic vesicle
,
[+]
|
krt12.4
|
|
tropicalis
|
NF stage 14
|
epidermis
|
|
|
Fig 3. Knockdown of nol11 disrupts cranial neural crest development.
At stages 14 and 24 neural and CNC development appears normal in one side treated embryos, as assayed by expression of key marker genes including sox3, cytokeratin, twist and slug. By stage 28 however, reductions are apparent in the expression of numerous CNC genes. The branchial arches are also smaller on the MO treated side at stage 28. Graph displays number of embryos exhibiting abnormal gene expression in control and nol11 morphant embryos.
doi:10.1371/journal.pgen.1005018.g003 |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
not
|
|
tropicalis
|
NF stage 28
|
forebrain
,
telencephalon
,
branchial arch
,
chordoneural hinge
,
pineal gland
,
[+]
|
hoxb3
|
|
tropicalis
|
NF stage 28
|
presomitic mesoderm
,
rostral presomitic mesoderm
|
sox3
|
|
tropicalis
|
NF stage 28
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
spinal cord
,
[+]
|
pax2
|
|
tropicalis
|
NF stage 14
|
pre-chordal neural plate
,
neural crest
,
cranial neural crest
,
optic field
,
anterior neural ridge
|
pax2
|
|
tropicalis
|
NF stage 28
|
otic vesicle
,
hindbrain
|
ap2a1
|
|
tropicalis
|
NF stage 14
|
neural crest
,
cranial neural crest
|
myod1
|
|
tropicalis
|
NF stage 14
|
mesoderm
,
presomitic mesoderm
,
paraxial mesoderm
|
|
|
S3 Fig. Patterning and Apoptosis in nol11 morphants.
A) Somite, CNC and neural development appear intact in nol11 morphants at stage 14 as assayed by myoD, ap2 and pax2 expression. Expression of xnot appears reduced at stage 28. B) Reduced dlx5 expression and BA hypoplasia on treated side of a stage 28 embryo. Neural patterning is normal at this stage as assayed by expression of pax2, hoxb3 and sox3. C) Whole mount TUNEL staining of treated and untreated sides of a stage 28 embryo. Note the increased staining in the craniofacial regions of the morphant side.
doi:10.1371/journal.pgen.1005018.s003 |