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FIG. 4. Misexpresslon of Xwnt-8 in the dorsal mesoderm results in the loss of both forebrain mad mldbram. Untreated embryos (A-C) or embryos
made to misexpress Xwnt-8 in the dorsal mesoderm during gastrulation (D-I) were cultured to tailbud stage and hybridized with digoxygeninlabeled
X-dll3, Xwnt-8b, and Xotx-2 probes as indicated above each column. All embryos are oriented with anterior to the left, and dorsal toward
the top of the figure. (A) X-dlI3 staining of branchial arches (small black arrows), ouc (large black arrow) and olfactory (black arrowhead) placodes,
telencephalon (open black arrow), and ventral diencephalon (open white arrow) is indicated. Staining of the ventral diencephalon is partially out
of the plane of focus in this figure and thus appears reduced relative to that shown in Fig. 2A. (B} Arrow denotes staining of the dorsal diencephalon
and arrowhead indicates staining of cells at the forebram-midbram boundary following hybndizatlon with an Xwnt-Sb probe. (C) Xotx-2 staining
of the telencephalon (open black arrow), and the rnidbrain (between filled arrows) is indicated. |
