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dlx5xenopus   

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Experiment details for dlx5

Murato Y and Hashimoto C (2009) Assay

Xhairy2 functions in Xenopus lens development by regulating p27(xic1) expression.

Gene Clone Species Stages Anatomy
dlx5.L laevis NF stage 11.5 ectoderm , ventral
dlx5.L laevis NF stage 12 ectoderm , ventral
dlx5.L laevis NF stage 12.5 ectoderm , ventral , anterior

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  Figure 1. Xhairy2 is expressed in PPE from gastrula stage. WISH analyses of Xhairy2 expression from mid to late gastrula stage in comparison with PPE-marked dlx5. a-i': Xhairy2 expression. a, d, g: Dorsal views with anterior side down. b, e, h: Anterior views with dorsal side up. White rectangles in b, e, and h indicate PPE expression. c, f, i: Mid-sagittal views with anterior side to the right. Embryos were halved and the left halves are shown. c', f', i': High-magnification views of areas enclosed by black rectangles in c, f, and i, respectively. White brackets indicate PPE expression in the sensorial layer, not in the superficial layer. j-r': dlx5 expression. Samples are shown in the same way as a-i'.

Gene Clone Species Stages Anatomy
dlx5.L laevis NF stage 15 ectoderm , ventral , anterior

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  Figure 4. Xhairy2 knockdown reduces the expression of LF marker genes in neurulae without marked changes in PPE marker expression. Micro-injection and lineage tracing were performed as described in Figure 3. Injected MO is indicated at the upper right corner and WISH probe is indicated at the lower right corner of each panel. a: Schematic presentation of LF in neurulae. b-i: LF marker genes. b, c: Two-color WISH of st.-15 embryos to confirm LF position. LF expression (black arrowheads) of pax6 (magenta) and six3 (purple) is indeed outside of the neural plate border that is marked by Xhairy2 (light blue). j, k: Embryos were injected with 6.9 ng of Xhairy2 MO and/or 20 pg of Xhairy2b mRNA where Xhairy2 MO does not bind in order to check the specificity of Xhairy2 MO to LF-loss phenotypes. I: Quantitative summary of j and k. Error bar: standard deviation of 5 independent experiments. m-t: PPE marker genes. Red arrowheads indicate reduction of marker gene expression. All the samples were pictured from the anterior side with the dorsal side up.