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Experiment details for dse

Gene expression of the two developmentally regulated dermatan sulfate epimerases in the Xenopus embryo.

Gene expression of the two developmentally regulated dermatan sulfate epimerases in the Xenopus embryo.

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Gene Clone Species Stages Anatomy
dse.L laevis NF stage 27 forebrain

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  Fig 4. Dse is expressed in the eye, migratory neural crest and the hypochord. Embryos are shown in lateral view (A,D-E,H-J’), and transversally sectioned through the trunk (B-C) and head (F,G). (A) Embryo at stage 25. The bold line indicates the level of the section in B. (B,B’) Dse is expressed in the epidermis, notochord and hypochord. The arrowhead shows Dse transcripts in trunk neural crest cells adjacent to the spinal cord. (C) Matching section of a sibling embryo showing Twist1 expression in trunk neural crest cells (arrowhead). (D-I) Tailbud embryos. The bold lines indicate the level of sections in F and G. Dse is expressed in the forebrain, proximal eye and Twist1+ cranial neural crest (indented arrowheads). The numbers indicate the mandibular (1), hyoid (2) and branchial neural crest (3,4). (J,J’) Embryo at stage 31 after removal of the epidermis. Dse is expressed in the spinal nerves (arrow) and migrating trunk neural crest cells (arrowhead). cg, cement gland; epi, epidermis; ey, eye; fb, forebrain; hc, hypochord; no, notochord; sp, spinal cord; so, somite. Scale bars are 500 μm (A,D-J) and 100 μm (B-C,J’).

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Gene Clone Species Stages Anatomy
dse.S laevis NF stage 27 forebrain

  dse (dermatan sulfate epimerase) gene_symbol (gene_name) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, head and anterior trunk region, lateral view, anterior left, dorsal up.

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Gene Clone Species Stages Anatomy
dse.L laevis NF stage 32 forebrain

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  Fig 9. Comparison of Dse and Dsel expression in the head. Embryos at stage 32 are shown in lateral views (A,E) and transversal sections (B-D’, F-H’). (A) Dse expression. The bold lines indicate the level of sections in B-D. (B-D’) Dse expression is found in the cranial neural crest (indented arrowheads). Note ventral expression domains in the forebrain, posterior midbrain, hindbrain, and notochord. Weak expression is visible in the trigeminal ganglia. (E) Dsel expression. The bold lines indicate the level of sections in F-H. (F-H’) Dsel expression appears in the cranial neural crest (indented arrowheads) and trigeminal ganglia. Note expression domain in the prechordal mesendoderm. Apparent are low-level transcripts in the entire ventral brain and robust bilateral expression domains in the ventral marginal zone of the mid- and hindbrain (arrows). fb, forebrain; hb, hindbrain; he, heart; mb, midbrain; no, notochord; pm, prechordal mesendoderm; V, trigeminal ganglion. Scale bars are 1 mm (A-H) and 200 μm (B’-H’).

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Gene Clone Species Stages Anatomy
dse.L laevis NF stage 33 and 34 forebrain

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  Fig 5. Dse is expressed in the adenohypophysis, melanophores, dermomyotome and sclerotome. Embryos are shown in lateral view (A,A’,F), dorsal view (G), transversal sections (B-D’,H,I) and horizontal section (E). (A,A’) Embryo at stage 33. The bold lines indicate the level of sections in B-E. (B-E) Vibratome sections showing Dse expression in the adenohypophysis, forebrain, ventral midbrain, epidermis, notochord, hypochord, dermomyotome, and sclerotome. Note also Dse transcripts in cranial neural crest cells (indented arrowheads) and melanophores (filled arrowhead). (F-I) Embryo at stage 40. The bold lines indicate the level of sections in H and I. Dse is expressed in post-migratory cranial neural crest cells (indented arrowheads) and the notochord. ad, adenohypophysis; dm, dermomyotome; epi, epidermis; ey, eye; fb, forebrain; hc, hypochord; he, heart; mb, midbrain; no, notochord; sc, sclerotome; so, somite; sp, spinal cord. Scale bars are 1 mm (A,A’,F-H) and 100 μm (B-E,I).

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Gene Clone Species Stages Anatomy
dse.S laevis NF stage 33 and 34 forebrain

  dse (dermatan sulfate epimerase) gene_symbol (gene_name) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 33 & 34, head and anterior trunk region, lateral view, anterior left, dorsal up.
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