||Fig 4. Dse is expressed in the eye, migratory neural crest and the hypochord.
Embryos are shown in lateral view (A,D-E,H-J’), and transversally sectioned through the trunk (B-C) and head (F,G). (A) Embryo at stage 25. The bold line indicates the level of the section in B. (B,B’) Dse is expressed in the epidermis, notochord and hypochord. The arrowhead shows Dse transcripts in trunk neural crest cells adjacent to the spinal cord. (C) Matching section of a sibling embryo showing Twist1 expression in trunk neural crest cells (arrowhead). (D-I) Tailbud embryos. The bold lines indicate the level of sections in F and G. Dse is expressed in the forebrain, proximal eye and Twist1+ cranial neural crest (indented arrowheads). The numbers indicate the mandibular (1), hyoid (2) and branchial neural crest (3,4). (J,J’) Embryo at stage 31 after removal of the epidermis. Dse is expressed in the spinal nerves (arrow) and migrating trunk neural crest cells (arrowhead). cg, cement gland; epi, epidermis; ey, eye; fb, forebrain; hc, hypochord; no, notochord; sp, spinal cord; so, somite. Scale bars are 500 μm (A,D-J) and 100 μm (B-C,J’).