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enc1.2xenopus   

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Experiment details for enc1.2

Peyrot SM et al. (2011) Assay

A revised model of Xenopus dorsal midline development: differential and separable requirements for Notch and Shh signaling.

Gene Clone Species Stages Anatomy
enc1.2.L laevis NF stage 10.5 to NF stage 11 dorsal marginal zone , upper blastopore lip , presumptive axial mesoderm
enc1.2.L laevis NF stage 14 pre-chordal neural plate , notochord , axial mesoderm , floor plate , neural tube

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  Fig. S6. Analysis of floor plate markers reveals two separate populations of medial floor plate. In situ hybridization for indicated markers was performed at stage 10.5–11 (A, C, E, G, I, K, M) and neurula stages (st.14—B, D, F, J, L, N, O; st.16—H, P). Prime panels (B', etc.) show transverse bisections of matching neurula stages (notochord outlined in red). Markers expressed in the organizer (marked by asterisk) at gastrula stages are expressed throughout the dorsal midline and in a narrow domain in the floor plate (A–N') at neurula stages. Netrin expression begins at early neurula stages (st.14, O) in a wider domain than early markers (O', P' compared to B'–N'). We have designated the narrow, early-forming FP the 1° MFP and the wider, later-forming FP the 2° FP.

Gene Clone Species Stages Anatomy
enc1.2.L laevis NF stage 10.5 to NF stage 11 dorsal marginal zone , upper blastopore lip , presumptive axial mesoderm

  enc1.2 (ectodermal-neural cortex (with BTB-like domain), gene 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 10.5, vegetal view, dorsal up.

Gene Clone Species Stages Anatomy
enc1.2.L laevis NF stage 14 pre-chordal neural plate , notochord , axial mesoderm , floor plate , neural tube

  enc1.2 (ectodermal-neural cortex (with BTB-like domain), gene 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 14, dorsal view, anterior up.