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epha4xenopus   

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Experiment details for epha4

Rothe M et al. (2017) Assay

An Epha4/Sipa1l3/Wnt pathway regulates eye development and lens maturation.

Gene Clone Species Stages Anatomy
epha4.L laevis NF stage 10 dorsal marginal zone
epha4.L laevis NF stage 13 neural crest , optic field , preplacodal ectoderm , presumptive rhombomere
epha4.L laevis NF stage 14 neural plate , migratory neural crest cell , optic field , anterior placodal area , presumptive rhombomere
epha4.L laevis NF stage 20 optic vesicle , cranial placode , migratory neural crest cell , presumptive rhombomere
epha4.L laevis NF stage 29 and 30 to NF stage 35 and 36 lens , forebrain , pronephric nephrostome , tail bud , eye , [+]
epha4.L laevis NF stage 37 and 38 lens , retina , neural crest , pronephric nephrostome , eye , [+]

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  Fig. 1. Comparative gene expression of sipa1l3 and epha4. (A) WMISH shows that sipa1l3 and epha4 are similarly expressed in the early eye field (white arrowheads). Scale bar: 500 µm. (B) sipa1l3 and epha4 are expressed in the developing eye (white arrowheads) including the retina (black arrowheads) and lens (red arrowheads). Dashed lines in B, stage 37 indicate level of transversal sections. Scale bars: 500 µm in stages 30/35; 250 µm in stages 35/37.

Gene Clone Species Stages Anatomy
epha4.L laevis NF stage 13 neuroectoderm , pre-chordal neural plate , neural groove , preplacodal ectoderm , presumptive rhombomere

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  Figure S4: Comparative expression of epha4 and rax At stages 13 and 14, epha4 and rax showed a partial overlapping expression in the early eye field (white arrowheads). Scale bar: 500µm.