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Fig. 8. X-Delta-2 MO effect on upstream regulators of Hox genes. Non-injected controls (Nic; A,C,E,G) and embryos injected on the left-hand side with X-Delta-2 MO (B,D,F,H) were analysed at gastrula stage for the expression of Xbra (A,B: 89%, n=27) and BMP-4 (C,D: 68%, n=31), and at neurula stage for the expression of Xcad-3 (E,F: 75%, n=28) and FGF-4 (G,H: 90%, n=32) by whole-mount in situ hybridisation. X-Delta-2 MO downregulates the expression of BMP-4 and Xcad-3, without affecting the expression of Xbra and FGF-4. Embryos at gastrula stage are shown from the vegetal side, dorsal up (A–D), and at neurula stage from the dorsal side, anterior up (E,F) or posterior side, dorsal up (G,H). The arrowhead indicates the injected side of the embryo. |
