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fn1 (fibronectin 1) gene expression in Xenopus laevis embryo, assayed via immunohistochemistry, NF stage 28, lateral view, anterior left, dorsal up. |
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Figure 6. Divergent, gene-specific somite defects in Dvl morphants
Lateral confocal projections displaying somite morphology of Dvl morphants with a muscle specific mouse anti-12/101 antibody (green) and the somite boundaries with a mouse anti- fibronectin antibody (red). (A): Normal morphology of the medial flank of a stage 29/30 embryo (B): Normal fibronectin deposition in somite boundaries stage 29/30 embryo. (C): Dvl1 morphant stage 29/30 embryo displaying segmentation defect (white arrow) (D): Dvl1 morphant stage 29/30 embryo displays defects in the deposition of fibronectin (red) while hevronshape is conserved. (E): Dvl3 morphant stage 29/30 embryo displaying un- hevronedsomites and multiple cells are disrespecting the established somite boundaries. (F): Dvl3 morphant stage 29/30 embryo displaying normal deposition of fibronectin between un-hevronedboundaries. Somite defects were observed in 50-90% of morphants, depending upon the experiment. |