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Experiment details for fn1

Gray RS et al. (2009) Assay

Diversification of the expression patterns and developmental functions of the dishevelled gene family during chordate evolution.

Gene Clone Species Stages Anatomy
fn1.S laevis NF stage 28 to NF stage 29 and 30 intersomitic region

  fn1 (fibronectin 1) gene expression in Xenopus laevis embryo, assayed via immunohistochemistry, NF stage 28, lateral view, anterior left, dorsal up.

Gene Clone Species Stages Anatomy
fn1.S laevis NF stage 29 and 30 intersomitic region

  Figure 6. Divergent, gene-specific somite defects in Dvl morphants Lateral confocal projections displaying somite morphology of Dvl morphants with a muscle specific mouse anti-12/101 antibody (green) and the somite boundaries with a mouse anti- fibronectin antibody (red). (A): Normal morphology of the medial flank of a stage 29/30 embryo (B): Normal fibronectin deposition in somite boundaries stage 29/30 embryo. (C): Dvl1 morphant stage 29/30 embryo displaying segmentation defect (white arrow) (D): Dvl1 morphant stage 29/30 embryo displays defects in the deposition of fibronectin (red) while hevronshape is conserved. (E): Dvl3 morphant stage 29/30 embryo displaying un- hevronedsomites and multiple cells are disrespecting the established somite boundaries. (F): Dvl3 morphant stage 29/30 embryo displaying normal deposition of fibronectin between un-hevronedboundaries. Somite defects were observed in 50-90% of morphants, depending upon the experiment.