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FIG.5 Whole mount in situ hybridization with XFKH2. Embryos were probed with the anti-sense XFKH2 probe with the exception ofF which was
probed with the sense XFKH2. The embryos were fixed at stage 11 (A), stage 18 (B, G, and H), stage 26 (C), and stage 34 (D, E, F, and 1). G is a stage 18
embryo ventralized by uv. H (stage 18) and I (stage 34) were dorsalized by LiCl treatment. In A, Vindicates the ventral side and D the dorsal side. The
notochord is designated nc in B, proctodeum by pd in D, and the floor plate by fp, the hypochord by he, and the foregut near the developing liver
diverticulum by fg in E. All hybridizations were done to albino embryos. |
