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Fig.3. Cadherin expression and sorting. (A–D) Mixed and reaggregated ectoderm cells at the neurula stage. C-cad-MO (10 ng/embryo) cells sort from C-cadherin or EPδC cells (B,D); C-cadherin and EPδC cells do not segregate (C), similar to controls (A). (E–G) Ventral ectoderm C-cadherin and C-cad-MO cells do not segregate in vivo (G), similar to controls (E); M-PAPC-injected cells separate from normal cells (F). (H–J) Chordamesoderm cell intercalation in the late gastrula. C-cadherin and C-cad-MO cells intercalate (J, arrowheads) as in controls (H, arrowheads). C-cad-MO cells are abnormally round (asterisk). Intercalation does not occur between M-PAPC-expressing and uninjected tissue (I). Nuclei were immunostained with FoxA4 (chordamesoderm) and MyoD (paraxial mesoderm) antibodies. Arrows indicate the notochord–somite boundary. (K–M) Explantation does not induce sorting. Inner ectoderm isolated from early gastrulae, cultured to the neurula stage. The C-cadherin–C-cad-MO boundary (M) resembles that of the negative (K), rather than positive (M–PAPC injection, L) control. Scale bars: 200 μm. |
