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Fig. 3. Spatial distribution of XFD-12' transcripts analyzed by whole-mount in situ hybridization. XFD-12' expression in different embryonic stages (as indicated) is visualized either in purple (A-H,K,L) or in brown (I,J,M,N), staining for XFD-1(Knoèchel et al., 1992) (I,J), En-2 (Hemmati-Brivanlou et al., 1991) (M) and Krox-20 (Bradley et al., 1993) (N) is shown in red. Expression of XFD-1 (I',J') is indicated in purple. Stained embryos were gelatin-embedded and vibratome-sectioned at 30 mm. Magnified regions are boxed and demarcated by the corresponding letter.
(A) Lateral view (bottom, vegetal pole; right, dorsal), (B-E) dorsal view (blastopore/posterior, bottom), (K-N) lateral view (posterior, right), (F,F') sagittal sections (orientated as in (a), (G,I,I') sagittal sections (top, dorsal; blastopore, right), (L',L'',M',N') sagittal sections (top, dorsal; anterior, left), (H,J,J') transverse sections within the posterior third of the embryo (top, dorsal). |
