|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
frzb
|
|
laevis
|
NF stage 10.5
|
upper blastopore lip
,
blastopore
,
dorsal
|
frzb
|
|
laevis
|
NF stage 15
to
NF stage 20
|
prechordal plate
,
endoderm
,
anterior
|
szl
|
|
laevis
|
NF stage 10.5
|
ventral marginal zone
,
lower blastopore lip
|
szl
|
|
laevis
|
NF stage 15
|
mesoderm
,
blastopore lip
,
ventral
|
szl
|
|
laevis
|
NF stage 20
|
circumblastoporal collar
,
heart primordium
,
ventral mesoderm
|
sfrp2
|
|
laevis
|
NF stage 10.5
|
neuroectoderm
,
upper blastopore lip
,
dorsal
|
sfrp2
|
|
laevis
|
NF stage 15
|
forebrain
,
neuroectoderm
,
chordal neural plate
,
neural plate
,
neural tube
,
[+]
|
sfrp2
|
|
laevis
|
NF stage 20
|
somite
,
eye
,
lateral plate mesoderm
,
trunk
,
anterior
,
[+]
|
|
|
Fig. 3. Expression of crescent, frzb-1, sfrp-2 and sizzled in Xenopus embryos by whole-mount in situ hybridization. (A–D) Early gastrulae (stage 10.5) in vegetal (A,B,D) and lateral (C) view. crescent, frzb-1and sfrp-2 are expressed in the dorsal and sizzled in the ventral blastopore lip. Note an additional expression domain of sfrp-2 in the prospective dorsal neuroectoderm (ne). (E–H) Neurulae (stage 15) in dorsal (E–G) and ventral (H) view. crescent and frzb-1 transcripts are localized to anterior endoderm and prechordal plate at this stage. sfrp-2 expression is strong in the anterior neural plate (forebrain and eye field) and posterior neural plate (hindbrain and spinal cord), and is absent from the floor plate. sizzled mRNA is expressed in the ventral mesoderm (vm), including ventral blood islands, and the ventral part of the closing blastopore lip (vb). (I–L) Tailbud embryos (stage 20) in lateral view. The crescent and frzb-1 expression domains overlap in the prechordal plate and pharyngeal endoderm. Note the additional crescent domain in the pronephros (pn). sfrp-2 has a complex expression pattern in eyes, neural tube, somites and in a belt-like anterior part of the trunk. sizzled transcripts are located in ventral mesoderm and in developing heart (he). |