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Fig. 2. Expression of Xenopus crescent gene. (A–C,E,G) Localization of Xenopus crescent mRNA was visualized by whole-mount in situ hybridization. Embryos were treated with benzyl benzoate/benzyl alcohol to become transparent. (D,F,H) Sections of stained embryos shown in bright field (left) and DAPI staining (right). (A) Vegetal view of early gastrula. (B) Lateral view of mid-gastrula. (C,E) Dorsal (left) and lateral (right) view of neurula. (G) Lateral view of tailbud stage embryo. (D,F) Sagittal section of neurula. (H) Transverse section of early tailbud stage embryo. Numerical figures indicate developmental stages (Nieuwkoop and Faber, 1967). Arrowheads indicate the dorsal blastopore. A, anterior; D, dorsal; en, endoderm; lp, lateral plate; nc, notochord; ne, neuroectoderm; p, pronephros; pcp, prechordal plate. |
