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Fig. 2. In situ hybridization of frzb-1 and crescent mRNA during neurula and early tailbud stages. (A-H′) In situ hybridization of frzb-1 (A-D) and crescent (E-H) during neurula and early tailbud stages. frzb-1 and crescent are stained purple/blue, the cement gland marker (XCG), dark red and the CNS-specific marker (nrp1), light orange. Arrows indicate the presumptive primary mouth. cg, cement gland. A-H are frontal views, scale bars: 200 μm; A′-H′ are sagittal sections with anterior to the left, scale bars: 130 μm. |
