|
Fig. 3. Whole mount in situ expression of xfz2. Whole mount in situ hybridization was performed on albino gastrulae (stage 12; A), early neurulae (stage 16; B,F), late neurulae (stage 22; C, G), tailbud (stage 26; D, H) and tadpole (stage 31; E, I). Stage and view are indicated in each panel. pm presomitic mesoderm, dl dorsal lip, so somite, ea eye anlage, ev eye vesicle, cg cement gland, sc spinal cord, ba branchial arches. Brackets in E indicate the enlarged area in I. Arrows in I indicate the somite boundaries. Xfz2 sense control probes performed in parallel have no staining and a highly similar frizzled (xfz7) has a very different expression pattern (not shown) indicating specificity of xfz2 staining. |