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Fig. 3. Wnt11 is expressed adjacent to the migrating neural crest. Simple and double in situ hybridization were performed for the neural crest marker gene Slug, Wnt11 and Fz7, as indicated at the top of the figure. (A-D) Dorsal (A,B) and lateral (C,D) views of stage 16-17 embryos; dashed lines indicate the sections shown in E-H; white arrow, weaker Wnt11 expression; black arrow, stronger Wnt11 expression. (E-H) Sections of the embryos shown in A-D. Purple arrowhead indicates the region where Wnt11 is detected. n, notochord; bracket, region of Slug and Fz7 expression; dashed line marks the endomesoderm. (I-K) Dorsal (I,J) and lateral (K,L) views of stage 23 embryos. e, eye; purple arrowhead indicates the region where Wnt11 is detected; green arrowhead shows the three streams of migrating neural crest. (M,N) Summary of Slug, Fz7 and Wnt11 expression. (M) Premigratory stages. Wnt11 is expressed in the ectoderm adjacent to the neural crest just before migration starts. A subpopulation of the neural crest cells expresses Fz7. (N) Migratory stages. During neural crest migration, Wnt11 is expressed next to the migrating neural crest. np, neural plate; nc, neural crest; s, somite; n, notochord; nt, neural tube; e, epidermis. |
