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Fig. 3. Cells from the neural crest transplants migrate to cartilage-forming areas in Tbx6 knockdown embryos. (A, B) Wnt11 and Fz7 were expressed in the cranial neural crest cells and adjacent ectoderm indicated by arrowheads in stage 17 embryos injected with Tbx6 exint-MO as revealed by whole-mount in situ hybridization. Dorsal view, anterior to top. MO-injected sides (*) are shown by Magenta-GAL staining. (C) Schematic diagram of the cranial neural crest-transplantation experiment. Distributions of fluorescent cranial neural crest cells in stage 27 (D) and stage 28 (E) host embryos. Both images are flipped horizontally. |
