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Fig. 1. Endogenous expression of fzd7 in Xenopus heart and relative to wnt11 and
wnt11-R expression. (A, B): stage 10.5 (mid-gastrula) fzd7 and wnt11 expression
detected at the dorsal side of the embryo and appear complementary in the
presumptive heart region. (C-Cii and D-Di): fzd7 and wnt11-R expression at stage 25.
fzd7 is seen in the heart field and wnt11-R in the anterior endoderm. fzd7 and wnt11-
R expression are complementary in the heart region (Cii, Dii). (E, Ei and F, Fi): stage
29 embryos with fzd7 and wnt11-R expression in the heart field. hf: heart field, ae:
anterior endoderm. Magnification 20x. |
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Fig. 2. fzd7 expression coincides with expression of the early heart markers nkx2-5,
tnnic and gata6. Lateral view of Xenopus laevis embryos at stage 31 showing fzd7
expression detected in red (A-Aii) and co-localised by double in situ hybridisation with
other heart markers in dark blue including nkx2-5 (B-Bii), tnnic (C-Cii) and gata6 (DDii).
(Ai, Bi, Ci, Di). Magnified lateral view of the same embryos in (A, B, C and D)
respectively. (Aii, Bii, Cii, Dii). Cross section through the heart region of the embryos
in (A, B, C, D) respectively. fzd7 is expressed in the myocardium and pericardium (Aii)
and in other structures including neural crest, eye, pronephric duct and tail bud. fzd7
expression shows a high degree of overlapping with the heart markers in the
myocardium but not in the pericardium (Bii, Cii, Dii). h: heart, c: cement gland, e: eye,
nc: neural crest, pnd: pronephric duct, tb: tail bud, mc: myodcardium, lpm: lateral plate of mesoderm. Magnification: 20x in (A, B, C, D), 30x in (Ai, Bi, Ci, Di), 200x in (Aii, Bii,
Cii, Dii). |
