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fzd7xenopus thyroid primordium [+] 

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Experiment details for fzd7

Spagnoli FM and Brivanlou AH (2008) Assay

The Gata5 target, TGIF2, defines the pancreatic region by modulating BMP signals within the endoderm.

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Gene Clone Species Stages Anatomy
fzd7.L laevis NF stage 29 and 30 thyroid primordium

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  Fig. 2. Whole-mount in situ hybridization of selected array clones. (A) Xenopus embryos at early gastrula (st. 10), tailbud (st. 30) stage and dissected whole gut (st. 43) were hybridized with antisense probe for Fz7/clone 10B11. Arrowheads indicate expression in the endoderm; the dotted line depicts the dorsal blastopore lip. Staining in endodermal derivatives, such as the hepatic and pancreatic regions, is indicated by a bracket in the middle panel. (B) Embryos at early gastrula (st. 10), tadpole (st. 35) stage and dissected whole gut (st. 43) were hybridized with HDGF/clone 7C5. Arrowheads point to expression in the endoderm; and the bracket in the middle panel indicates endodermal derivatives, such as the hepatic and pancreatic regions. (C) Embryos at gastrula (st. 10.5), tadpole (st. 37) stage and dissected whole gut (st. 42) were hybridized with clone 7G9 [EST similar to hypothetical protein (BG410109)] indicating expression in the ectoderm and hepatic rudiment (bracket in the middle panel). (D) Embryos at gastrula (st. 10), tadpole (st. 36) stage and dissected whole gut (st. 42) were hybridized with clone 8C11 [EST similar to hypothetical protein KIAA0592 (BG410148)] showing expression in the dorsal ectoderm, brain, pronephros, liver and duodenum. (E) Embryos at gastrula (st. 10), early tailbud (st. 27) stage and dissected whole gut (st. 42) were hybridized with clone 7B7 [EST similar to hypothetical protein (BC094159)] indicating expression in the ectoderm, brain, branchial arches and pancreas. (F-J) In situ hybridizations on serial sections of gastrula and neurula stage embryos using Xenopus TGIF2 (xTGIF2) and Gata5 probes show expression of both genes in a subset of endodermal cells. xTGIF2 is also strongly expressed in the ectoderm and mesoderm. (F,F′) In situ hybridization on section of stage 10 embryo using antisense probe for TGIF2/clone 8B1 (* indicates the dorsal side). Red dashed box outlines the TGIF2-positive endodermal region, which is magnified in F′. Arrowheads point to endodermal cells. (G) Gata5 in situ hybridization on section of stage 10 embryo. Arrowheads point to endodermal cells. (H,H′) In situ hybridization on section of stage 10.5 embryo shows enriched staining in dorsal endodermal cells (* indicates the dorsal side). Red dashed box outlines the TGIF2-positive endodermal region, which is magnified in H'. Arrowheads point to endodermal cells. (I,I′) In situ hybridization on neurula stage whole (I) and sectioned (I′) embryos shows TGIF2 staining in anterior endodermal cells (red dashed box in I′). (J) Gata5 in situ hybridization on section of stage 17 embryo. (K) Dissected whole gut (st. 43) stained with TGIF2 probe shows expression in the pancreatic rudiment. a, anterior; d, duodenum; lv, liver; pa, pancreas; p, posterior.
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