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Fig. 8. Pax7 and Pax3 regulate mid–hindbrain boundary maintenance. Pax7 depletion leads to forebrain–midbrain expansion as seen by otx2 posterior expansion (B, C). This is accompanied by loss or posterior shift of the mid–hindbrain boundary (Gbx2, G, H) and En2 (L, M). A similar phenotype is observed after PaxEnR (D, I, N). Pax3 morphants do not exhibit expanded otx2 domain (E, yellow arrow indicates similar posterior boundaries on each side), but they also display defective or shifted mid–hindbrain boundary (J, O). Red arrows indicate the extent of shift between the posterior boundary on control non-injected (left) side, and the shifted boundary on injected (right) side. |
