| |
FIG. 3. Ectopically expressed Xgsk-3 disrupts expression of anterior and lateral genes. Embryos were injected in one cell at the two-cell
stage with Xgsk-3FS (E) or Xgsk-3 (A–D, F–H) and b-galactosidase RNA, cultured until stage 13.5 (A) or stage 14 (B– H), and stained for
b-galactosidase activity and the indicated gene expression. (A and B) XAG-1 expression in embryos viewed from the dorsal side. Anterior
is facing to the left in (A) and to the right in (B), with the injected sides facing down. Note the presence of b-galactosidase activity and
the absence of XAG-1 expression within the neural plate region (arrowhead) in (B). Although not apparent in the photograph, the neural
folds, which mark the borders of the neural plate, are readily seen with a microscope. (C and D) Xotx2 expression on the uninjected (C)
and injected (D) sides of one embryo. Note anterior (arrow) and posterior (arrowhead) regions of expression in (C). In the embryo in (D),
only the anterior region of expression has expanded. (E and F) Anterior-lateral view of XANF-2 expression in embryos injected with Xgsk-
3FS (E) and Xgsk-3 (F) RNA. Note the appearance of anterior (arrow) and posterior (arrowhead) regions of expression in (E). In the embryo
in (F), both the anterior and posterior regions of expression have expanded on the injected side (facing down). (G and H) Xgbx-2 expression
on the uninjected (G) and injected (H) sides of one embryo. The epidermal expression is visible in (G). The neural domain of expression
is mostly hidden in this view by the neural folds. The pairs of embryos in (C and D) and (G and H) are oriented with dorsal at the top
and anterior facing the center. Staining for b-galactosidase activity is visible in the microscope although it is not visible in all photographs. |
