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Fig 1
RARγ1 is required for the expression of mesoderm markers. (A-D) Embryos were microinjected unilaterally at the 2-cell or 4-cell stage with 3.3 ng RARγ1.S+3.3 ng RARγ1.L/S MOs (Fig. S3). Injected side is to the right of the dashed line, and is indicated by the magenta β-gal lineage tracer. WISH shows that RARγ1 MOs result in the loss of T, fgf8, wnt8 and gdf3. (E-H) Embryos were microinjected unilaterally at the 2-cell or 4-cell stage with 6.6 ng RARα2 MOs (Janesick et al., 2013). Injected side is to the right of the dashed line, and is indicated by the magenta β-gal lineage tracer. RARα2 MOs did not affect T, wnt8 or gdf3 expression, but did produce loss of N-tubulin as previously published (Janesick et al., 2013). All embryos, except in H, are shown in vegetal view at stage 10.5/11. H is shown in dorsal view, with anterior on the bottom at stage 14. Fractions represent the portion of embryos displaying the phenotype. NC, no change. |
