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grem1xenopus   

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Experiment details for grem1



XPteg (Xenopus proximal tubules-expressed gene) is essential for pronephric mesoderm specification and tubulogenesis.

Gene Clone Species Stages Anatomy
grem1 xenopus NF stage 35 and 36 pronephric duct

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  Fig. 4. XPteg promotes pronephric mesoderm formation (A and B). Overexpression of XPteg stimulates the expression of Pax2, SMP30, Lim1 and Pax8 in a dose-dependent manner. Eight-cell stage embryos were injected with various doses of XPteg RNA ranging from 37.5 pg to 500 pg in the presumptive pronephric field and then in situ hybridized against these markers. Intermediate doses of XPteg RNA (125–250 pg) were optimal to activate pronephric marker expression without defects in lateral medoserm. Arrowheads point to ectopic enlarged proximal tubules. (C) XPteg has no effects on the proximal–distal patterning of pronephric tubules. Expression of Gremlin, a marker for distal and connecting tubules was not affected by overexpression or depletion of XPteg. (D) RT-PCR analysis showing the activatory effects of XPteg on pronephric marker expression. Ectodermal explants were dissected at stage 9 from XPteg RNA (1 ng)-injected embryos, incubated in media containing activin A (10 ng/ml) and all-trans retinoic acid (10−4 M) for 3 h, and then cultured for 3 days in Steinberg’s solution and analyzed by RT-PCR. (E) Quantification of the band signals shown in panel (D).