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Fig. 7. Profilin1 does not interfere with mesoderm induction. Embryos injected dorsally with Profilin1 (2 ng) or XProfilin1 MO (50 ng) show normal mesoendoderm and neural induction, but abnormal tissue localization due to gastrulation defects. No effects were observed with injections of the control MO (50 ng) after gastrulation defects. The phenotype induced by the XProfilin1 MO can be rescued by co-expression of δN-Profilin1 (50 pg). Injected embryos show normal expression of mesodermal Xbra, and dorsal Gsc and Otx2 at st 10.5, but exhibit an Xbra expression surrounding a large blastopore that does not close at st 12. Gsc expression in control embryos at st 13 is observed in anterior mesoendoderm far from the closed blastopore (arrowheads), but in Profilin or XProfilin1 MO-injected embryo remains trapped near the open blastopore. Otx-2 is expressed anteriorly in both mesodermal and overlying neural tissues in control embryos at st 13 but in Profilin or XProfilin1 MO-injected embryos, two separate Otx-2 expression domains are obvious. Sox-2 is expressed in the neural plate at st 14 (arrowheads), but in Profilin or XProfilin1 MO-injected embryos, Sox-2 expression is seen surrounding the open blastopore. |
