| |
Fig. 5. Molecular marker analysis of XHNF1 beta/EngR mRNA-injected embryos. As indicated, embryos were bilaterally injected at 4-cell stage either dorsally (4C/2D), or ventrally (4C/2V), and processed for in situ hybridization with probes for goosecoid (gsc), chordin (chd), Xnot2, Xnr3, Xwnt-8, Xbra, Xpo and Xvent-2,. Analysis was performed either at early neurula stage 12.5 (A-F; A,D-F, dorsal views; B,C, posterior views), or at gastrula stage 10.25-10.5 (G-T; vegetal view). Control embryos (wt) are also shown. Note absence of dorsal blastopore lip in B and C. |
