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Fig. 3. dkk1 is required for prechordal plate formation. (A,D,G) Uninjected controls, (B,E,H) Embryos injected with anti15 Ab into the blastocoel at blastula stage and (C,F,I) embryos injected radially at the four-cell stage with dkk1 mRNA. In situ hybridization with probes to XHex1 (A-C), XBlimp1 (D-F) and XNot2 + gsc (G-I). (A-F) Embryos from early neurula stage (stage 13) were cut sagittally; dorsal side up, anterior to the right. White arrowheads indicate the anterior and posterior limits of expression. (G-I) embryos from stage 13 are shown from the dorsal side, anterior side up. (J-L) The prechordal plates of control (J), anti15 Ab- injected (K), or dkk1 mRNA-injected embryos (L) are shown from gastrocoel, anterior side is up. Red arrows indicate the lateral limit of Shh expression. PCP, prechordal plate, NOT, notochord. |
