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Fig. 2. xbarr1 regulates CE movements of dorsal tissue with no effect on cell fates. (A) Gain- and loss-of-function phenotypes of xbarr1. (B) DMZ elongation assays. (C)
Quantitation of convergent extension of DMZ explants shown in (B). (D and E) Whole-mount in situ hybridization analysis. (A–E) Two blastomeres of four-cell stage embryos
were injected in the dorsal equatorial region as indicated with xbarr1 RNA (1 ng), xbarr1 MO (10 ng) and Co MO (10 ng) and then cultured to stage 26 (A), 10.25 for in vitro
elongation assays (B) or 10.5–14 for in situ hybridization (D and E). |
