| |
Fig. 5. Gene expression profiles at
the late gastrula stage are more
uniform. (A) Animal, vegetal, DMZ
and VMZ cells were isolated from
stage 12 embryos and analyzed by
RT-PCR and dot blot. (B) The gene
expression profile of each cell is
shown. In this figure cells with the
same expression pattern have been grouped together rather than being shown in order of
their position in a 96-well plate. (C) Gsc in situ hybridization on bisected embryos at
stage 11.5 (parasagittal section, dorsal towards left) and stage 13 (sagittal section, anterior
towards right, dorsal towards the top). At stage 11.5, Gsc is not detected in ectoderm by
in situ hybridization, but by stage 13 a domain of Gsc expression can be seen in the
neurectoderm (ec; arrow) overlying Gsc expression in the axial mesoderm (m). |
