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Fig. 4. Gene expression patterns in embryos injected with MO1. (A,B) Expression of Goosecoid is downregulated in response to MO1 (B), but its expression domain is unaltered. (C,D) Downregulation of Xbra in response to MO1 (D). See text for details. Embryos in A-D were fixed at similar morphological stages rather than at different times after fertilisation to compensate for slight delays in development. (E-H) The expression domains of Xvent-1 (E,F) and Xwnt-8 (G,H) are unaffected by MO1, even though their expression levels are very slightly (Xwnt-8; Fig. 3) or significantly (Xvent-1; Fig. 6) elevated. Embryos were injected at the two-cell stage, with 20 ng MO1 into each blastomere. |
