|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nkx3-3
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
mouth primordium
,
visceral pouch
,
cranial neural crest
,
cartilage element
,
head mesenchyme
,
[+]
|
emx2
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
otic vesicle
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
branchial arch
,
[+]
|
hand2
|
|
laevis
|
NF stage 33 and 34
to
NF stage 37 and 38
|
brain
,
pharynx
|
msx1
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
otic vesicle
,
eye
,
mouth primordium
,
mouth
,
pharynx
|
msx2
|
|
laevis
|
NF stage 33 and 34
to
NF stage 37 and 38
|
otic vesicle
,
mouth primordium
,
cranial placode
,
mouth
,
pharynx
,
[+]
|
nkx3-2
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
foregut
,
mouth primordium
,
mouth
,
cartilage element
,
head mesenchyme
|
|
|
Fig. S2.
Expression of emx2, nkx3-2-b, nkx3-3, hand1, hand2-a msx1-b, and msx2. Gene and stage are indicated in figure. Anterior to left in all lateral views, anterior to top in E, N, dorsal to top in II. For those genes with a section shown, the plane of section is shown as a red line on the corresponding whole-mount panel. (J and N) nkx3-3 is expressed in CNCCs where the primary jaw joint will eventually form (arrow). |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nkx3-3
|
|
laevis
|
NF stage 41
|
otic vesicle
,
joint
,
mouth primordium
,
mouth
,
lower jaw
,
[+]
|
hand2
|
|
laevis
|
NF stage 45
|
lower jaw
|
gdf5
|
|
laevis
|
NF stage 41
to
NF stage 45
|
joint
,
mouth primordium
,
mouth
,
lower jaw
,
joint region
|
nkx3-2
|
|
laevis
|
NF stage 41
to
NF stage 45
|
joint
,
mouth primordium
,
cranial placode
,
lower jaw
,
head mesenchyme
,
[+]
|
barx1
|
|
laevis
|
NF stage 45
|
lower jaw
|
|
|
Fig. 6. Gene expression in the intramandibular and primary jaw joint. Lateral views (A–C), dorsal views (I–M), and sections (E–G) are all shown with anterior to the left. Anterior views in (D and H). All black arrows mark the primary jaw joint, and all black arrowheads mark the intramandibular joint. (A–C) lateral views of whole-mount ISH for (A) nkx3-2-b, (B) nkx3-3, and (C) gdf5 at st. 41. Red lines depict the plane of section in the corresponding panel below each whole-mount photo, black arrows mark the future primary jaw joint, and the black arrowhead marks the future intramandibular joint. (D) Anterior view of whole-mount ISH for gdf5 at st. 45. (E–G) Sections of st. 41 larvae showing expressions in the future intramandibular and primary jaw joints. (E) nkx3-2-b is expressed in a broad domain surrounding the nascent primary jaw joint (black arrow), and also medially in the future infrarostral (white arrowhead) and future basihyal (white arrow). (F) nkx3-3 expression is found in the primary jaw joint (black arrow). (G) gdf5 expression is found in the nascent intramandibular joints (black arrowheads), and primary jaw joint (black arrow). (H) st. 45 whole-mount ISH for nkx3-2-a, shows expression in the primary jaw joint (black arrow). (I–L) dorsal views of st. 45 whole-mount ISH showing mandibular expression of (I) hand1, (J) barx1, (K) hand2-a+barx1 (double ISH; see Methods section), and (L) gdf5. Black arrowheads mark the future intramandibular joints. (M) Dorsal view with a ventral focal plane of a st. 48 X. laevis larva stained with alcian blue. Black arrowheads mark the intramandibular joints, black arrow marks the right primary jaw joint. (N and O) schematics of expressions showing hand2 (red), barx1 (green), and nkx3.2 (blue) in the mandibular arch. (N) wt and barx1 mutant zebrafish mandibular expression (adapted from Fig. 9B of Nichols et al., 2013). (O) st. 41 and st. 45 X. laevis mandible expression. The barx1 mutant zebrafish takes on a larval morphology strikingly similar to that of an anuran, with an extra joint forming within Meckel’s cartilage. Black arrowhead indicates the position of the future intramandibular joint. ir, infrarostral; mc, Meckel’s cartilage; pq, palatoquadrate. |