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FIGURE 6.
Ets1 physically interacts with HDAC1 through the ETS domain. A, co-IP was carried out with extracts of HEK293T cells transfected with either Ets1-Myc, HDAC1-HA separately, or both using antibodies against Myc or HA. B, co-IP indicates that Ets1-Myc binds to HDAC1-HA in Xenopus embryos. C–E, expression patterns of Xenopus hdac1 (C) and ets1 (D) in Xenopus embryo at stage 17. A schematic diagram of Xenopus hdac1 and ets1 expression (E) indicates overlap between them. F, Ets1 deletion mutants and their binding ability to HDAC1. G, co-IP was carried out to examine the binding of Myc-tagged Ets1 deletion mutants and HDAC1-HA in HEK293T cells. IB, immunoblot; TAD, transactivation domain; SAM, sterile α motif; PNT, pointed. |
